The House of Trastámara (Spanish: Casa de Trastámara) was a dynasty of kings in Spain, which first governed in Castile beginning in 1369 before expanding its rule into Aragon, Navarre and Naples. They were an illegitimate cadet line of the House of Ivrea.

The line of Trastámaran royalty in Castile ruled throughout a period of military struggle with Aragon. Their family was sustained with large amounts of inbreeding, which led to a series of disputed struggles over rightful claims to the Castilian throne. This lineage ultimately ruled in Castile from the rise to power of Henry II in 1369 through the unification of the crowns under Ferdinand and Isabella.

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